Investigations

5 Investigations visible to you, out of a total of 23

Euryhalin teleosts can live in freshwater as well as in seawater. The success of their survival depends then on their osmotic acclimation capacities. The objective of my work is to describe acclimation processes in the salted water at the rainbow trout by a study coupling functional genomic and genetic approaches. From a first differential gill transcriptomic study, a list of candidates genes was established. This study also allowed to investigate the physiological answer to a hyperosmotic kinetics
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-Le choix du système d'expression hétérologue ovocytaire a été fait afin de déorphaniser un maximum de récepteurs olfactifs identifiés chez l'adulte et la chenille de Spodoptera littoralis.
-Pour réaliser un screening moyen débit un robot de Two Electrode Voltage Clamp est utilisé (HiClamp, MultichanelSystem).

Afin de s'assurer de l'intégrité des plasmides pCS2+ injectés dans les ovocytes nous avons eu recours à un séquençage Sanger.

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lorem

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