PCR_TaqDNAPolymerase_SOP
Version 1

BuildDownload ZoomView content

>Utilisation de la TaqDNA Polymérase 5,000 U/ml NEB Biolabs
Préparation du Mastermix :
pour 1 réaction = Taq Ready Mix 12,5µl
Primer F 1,25µl
Primer R 1,25µl
ADN matrice qsp 0,05µg/µl
Eau DEPC qsp 25µl
>Programme du thermocycleur :
-Heat lid 105°C
-Dénaturation initiale 95°C 5minutes
30 cycles de PCR :
-Dénaturation 94°C 30secondes
-Hybridation 54°C 45secondes
-Elongation 72°C 2minutes
-Elongation terminale 72°C 5minutes

SEEK ID: https://research-sharing.cesgo.org/sops/4?version=1

Filename: PCR_TaqDNAPolymérase_SOP.pdf  Zoom Download

Format: PDF document

Size: 176 KB

Help
help Contributor and Creators
Activity

Views: 228   Downloads: 179

Created: 14th May 2018 at 08:07

Last updated: 14th May 2018 at 08:07

Last used: 15th Dec 2018 at 10:23

help Attributions

None

Related items

Powered by
Seek new full
(v.1.7.0)
Copyright © 2008 - 2018 The University of Manchester and HITS gGmbH